Cell Biology LabEnvironmental Toxin Assessment
EXAMPLE REPORT — FOR DEMONSTRATION PURPOSES ONLY · Based on IGL-Labor GmbH test modules · No real patient data is represented
CB
Cell Biology Lab
Environmental Toxin Assessment · CLIA Certified
Washington, Utah 84780, USA · cellbiologylab.com
Cell Biology Epigenetic Damage Marker Panel
White Blood Cell (PBMC) Intracellular Functional Analysis
Modeled after IGL-Labor GmbH (Wittbek, Germany) — Institute for Epigenetic Laboratory Diagnostics
Patient Name
Jane A. Sample
Date of Birth
March 14, 1978
Sex / Age
Female, 47
Order Number
CBL-2026-00143
Ordering Physician
Dr. W. Vosloo, ND
Requisition #
REQ-4472
Blood Collected
May 6, 2026
Date Received
May 7, 2026
Date Reported
May 9, 2026
Specimen
Whole blood (EDTA) → PBMC isolate
Result Summary — 44 Markers Assessed
11
Critical
8
Elevated
4
Borderline
12
Low
9
Normal
Clinical Priority: Multiple critical findings across DNA integrity (3 DNA adducts detected: AFB1, Lead, Lindane), immune sensitization (6 HIGH LTT responses including glyphosate, organophosphates, aflatoxin B), mitochondrial membrane (cardiolipin depletion, ANT blockade 46%), and heavy metal burden (aluminium 3x reference, elevated metallothionein). The pattern is consistent with multi-toxin chronic exposure with active DNA damage, mitochondrial dysfunction, and immune sensitization. Click any marker row to expand the clinical interpretation.
DNA Adducts & Genomic Integrity
Total DNA (Leucocyte)▼
82.42 µg/ml
ELEVATED
PCR & Fluorescence measurement (BCA protein assay)
DNA Adduct #1 — Aflatoxin B (AFB1-N7-Gua)▼
1.87 ng/ml
ELEVATED
PCR & Fluorescence measurement
DNA Adduct #2 — Lead (Pb-DNA complex)▼
5.46 ng/ml
CRITICAL
PCR & Fluorescence measurement
DNA Adduct #3 — Lindane (γ-HCH-DNA)▼
9.22 ng/ml
CRITICAL
PCR & Fluorescence measurement
DNA-Associated Zinc (Zn)▼
18.07 ng/ml
LOW
ICP-HRMS (Orbitrap, 60,000 res.)
Cell-Free DNA & Cell Death Markers
Cell-Free DNA (cfDNA) — Plasma▼
17.38 µg/dl
ELEVATED
PCR amplification (unmodified oligonucleotide primer, Tris-acetate-EDTA buffer) + BCA protein assay (Pierce 660nm)
LDH Total — Plasma▼
516.45 U/ml
ELEVATED
GS-FID (Gas chromatograph, capillary column)
LDH 5 (M4 isoform) — Plasma▼
98.16 %
CRITICAL
GS-FID
Superoxide Dismutase (SOD) Isoenzymes
Functional Red Cell SOD (Total)▼
52.63 U%
ELEVATED
PCR & Fluorescence measurement
SOD1 (Cu/Zn-SOD) — Cytoplasmic▼
188.36 U%
LOW
PCR & Fluorescence measurement
SOD2 (Mn-SOD) — Mitochondrial▼
152.73 U%
NORMAL
PCR & Fluorescence measurement
SOD3 (EC-SOD) — Extracellular▼
71.9 U%
ELEVATED
PCR & Fluorescence measurement
Glutathione System & Antioxidant Enzymes
Glutathione-S-Transferase (GST) — Serum▼
30.45 U/ml
NORMAL
GS-FID (Gas chromatograph, capillary column, Dimethylsiloxan, Fused Silica)
Glutathione-S-Transferase (GST) — Red Cells▼
124.45 U/ml
NORMAL
GS-FID
Glutathione (GSH) — Red Cells▼
2.36 mmol/l
NORMAL
GS-FID
Glutathione-Peroxidase (GSH-Px) Activity▼
23.81 U/g Hb
LOW
PHOT (photometric)
Erythrocytes (RBC Count)▼
4.36 Mio/µl
LOW
Cell counter (microscopy controlled)
Haemoglobin (Hb) Reference▼
21.18 g/dl
LOW
GS-FID
Glutathione GSH-Blood (Total)▼
792.54 µmol/l
NORMAL
GS-FID
Metallothionein & Heavy Metal Binding
Metallothionein (MT) — Functional Screening▼
494.7 µg/l
ELEVATED
ELISA assay screening & Fluorescence microscopy
Heavy Metal Distribution (Zn:Cu Ratio in MT)▼
30:70
BORDERLINE
ELISA assay screening & Fluorescence microscopy
Aluminium (Al) — Metallothionein-Associated▼
2.86 µmol/l
CRITICAL
ELISA assay screening & Fluorescence microscopy
Mitochondrial Membrane & Cardiolipin
Cardiolipin (CL) — Mitochondrial Membrane▼
14.09 %
LOW
Fluorescence microscopy (CL-specific fluorescent statin)
CL-Synthase Activity▼
77.72 U/10⁶ mt
LOW
Fluorescence microscopy
CL-Synthase Manganese Sites▼
6.18 No./10nM
LOW
Fluorescence microscopy
Mitochondrial Membrane Ca²⁺▼
204.15 nmol/l
ELEVATED
Fluorescence microscopy (Ca²⁺-sensitive fluorescent indicator)
Phosphatidylcholine (PTC) — Mitochondrial▼
95.81 U/10⁶ mt
LOW
Fluorescence microscopy
ATP Profile & Mitochondrial Energy
ATP^Mg+ (with excess Mg added)▼
1.27 nmol/10⁶
LOW
Enzymatic assay (phosphorylase kinase, PhK activity)
ADP-ATP Translocator — Decrease▼
54.15 change %
LOW
Enzymatic assay
Blocking of Active Sites (ADP-ATP)▼
46 %
CRITICAL
Enzymatic assay
L-Carnitine (Plasma)▼
27.01 µmol/l
NORMAL
Tandem MS (carnitine derivatives)
NAD / Niacin Status
Red Cell NAD (Nicotinamide Adenine Dinucleotide)▼
17.72 µg/ml
NORMAL
Purification with HPLC, Enzyme Analyser
Glycolytic Pathway Markers
Fructose-1,6-Bisphosphate (F-1,6-BP) — PFK1 Product▼
91.04 mmol/l
LOW
Spectrometry single λ continuous
Fructose-2,6-Bisphosphate (F-2,6-BP) — PFK2 Product▼
61.25 mmol/l
NORMAL
Spectrometry single λ continuous
Glucose-6-Phosphate Dehydrogenase (G6PD)▼
6.07 mmol/l
NORMAL
Spectrometry single λ continuous
Lymphocyte Sensitivity Test (LTT)
LTT — Aluminium (Al)▼
213 nmol/l
CRITICAL
Whole blood cytometric lymphocyte transformation test
LTT — Aflatoxin B▼
217 nmol/l
CRITICAL
Whole blood cytometric lymphocyte transformation test
LTT — Benzoquinone▼
215 nmol/l
CRITICAL
Whole blood cytometric lymphocyte transformation test
LTT — Lindane & Isomers▼
204 nmol/l
CRITICAL
Whole blood cytometric lymphocyte transformation test
LTT — Organophosphates▼
219 nmol/l
CRITICAL
Whole blood cytometric lymphocyte transformation test
LTT — Glyphosate▼
223 nmol/l
CRITICAL
Whole blood cytometric lymphocyte transformation test
LTT — Mercury Inorganic▼
126 nmol/l
BORDERLINE
Whole blood cytometric lymphocyte transformation test
LTT — Lead (Pb)▼
122 nmol/l
BORDERLINE
Whole blood cytometric lymphocyte transformation test
LTT — Formaldehyde▼
143 nmol/l
BORDERLINE
Whole blood cytometric lymphocyte transformation test
Methodology & References
DNA Adducts (Genomic DNA from Leucocytes)

PCR amplification with fluorescence measurement. Gene identification by sequence matching to NCBI database. DNA-associated zinc by ICP-HRMS. Method: PCR & Fluorescence measurement (BCA protein assay).

Cell-Free DNA & LDH Isoenzymes

cfDNA: Unmodified oligonucleotide primer, Tris-acetate-EDTA buffer, PCR amplification, BCA protein assay (Pierce 660nm). LDH isoenzymes: GS-FID, 5 tetramer isozymes (4H, 3Hm, 2H2M, H3M, 4M).

SOD Isoenzymes

Polymerase chain reaction (PCR) method & Fluorescence measurement. SOD1, SOD2, SOD3 isoenzyme-specific activity with gene-level DNA adduct biomarker assessment.

Glutathione System

GST activity: GS-FID (Gas chromatograph, capillary column, Dimethylsiloxan, Fused Silica). GSH-Px: PHOT (photometric, coupled NADPH oxidation). RBC count: cell counter, microscopy controlled.

Metallothionein & Heavy Metals

Metallothionein screening: ELISA assay & Fluorescence microscopy. Heavy metal distribution and aluminium: ICP-HRMS (Orbitrap, 60,000 resolution).

Mitochondrial Membrane & Cardiolipin

White cell mitochondria using phase-contrast, dark field & polarising microscopy. Cardiolipin: fluorescence-statin specific for CL. TL adducts: microplate array of fluorescence probes. Membrane pH and Ca2+ fluorescent indicators.

ATP Profile & Glycolysis

ATP profile: enzymatic assay (phosphorylase kinase, PhK activity), mixed leucocytes, whole cells. ADP-ATP translocator: mitochondrial TL assay. L-carnitine: Tandem MS. Glycolytic markers: Spectrometry single lambda continuous. NAD: HPLC + MST.

Lymphocyte Sensitivity Test (LTT)

Whole blood cytometric LTT. Microplate tech., Thermo FC Abs. Plate Reader, Twin-Line Triple-Quadrupol-MS, Capillary chromatographic (ISCC). Reference ranges represent a healthy population under non-challenged, non-provoked conditions.

Selected References
  1. IGL-Labor GmbH. Institute for Epigenetic Laboratory Diagnostics. Wittbek, Germany.
  2. Naviaux RK. Mitochondrion. 2014;16:7–17.
  3. Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine. 5th ed. 2015.
  4. Bussa S, Rumi C, Leone G, Bizzi B. J Clin Lab Immunol. 1993;40(1):39–46.
  5. Sies H et al. Nat Rev Mol Cell Biol. 2022;23:499–515.
DISCLAIMER: This is an example report for demonstration purposes only. Marker categories and methodologies are modeled after IGL-Labor GmbH (Wittbek, Germany) epigenetic laboratory diagnostics. No real patient data is represented. Reference ranges are based on published literature and IGL-Labor reference values. This report is intended for use by licensed healthcare practitioners only. Results should be interpreted in the context of clinical history and other diagnostic findings. Cell Biology Lab · Washington, Utah · cellbiologylab.com